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. 2019 Aug 22;4(16):e126982. doi: 10.1172/jci.insight.126982

Figure 8. cMSCs assist in promoting proplatelet formation and decrease platelet activation.

Figure 8

(A–C) Megakaryocytes from cMSC coculture group had decreased gene expression, as measured by real-time PCR, for SRC (A), DIAPH1 (B), and BAX (C), which are associated with increased megakaryocyte maturation (n = 3–4). (D and E) Furthermore, megakaryocytes from cMSC coculture group had decreased gene expression for LAT1 (D) and enriched for expression of RAP1B (E) relative to platelets from the control cultures, suggesting that cMSCs help to keep megakaryocytes in a lower activation state (n = 3–4). (F–H) Platelets from cMSC coculture group had increased protein expression, as measured by reverse phase protein analysis (RPPA), for IRF1 (F), STAT3 (G), and CCND3 (H), relative to platelets from the control cultures, suggesting that cMSCs are able to promote megakaryocyte maturation (n = 7–8). *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001. Two-tailed t tests were performed.