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. 2019 Aug 22;4(16):e129719. doi: 10.1172/jci.insight.129719

Figure 6. Lack of inflammatory reaction changes in the brain of TFEB-injected WT and PLP mice expressed under either the oligodendroglial or the neuronal promoter.

Figure 6

(A and B) Representative images (A) and quantification (B) of GFAP-positive astrocytic immunostaining in the SN of control, CMVie/hSyn-mTFEB–injected, and MBP-mTFEB–injected WT and PLP mice. Scale bar: 200 μm. (C and D) Representative images (C) and quantification (D) of GFAP-positive astrocytic immunostaining in the striatum of control, CMVie/hSyn-mTFEB–injected, and MBP-mTFEB–injected WT and PLP mice. Scale bar: 50 μm. (E and F) Representative images (E) and quantification (F) of Iba1-positive microglial cells immunostaining in the SN of control, CMVie/hSyn-mTFEB–injected, and MBP-mTFEB- injected WT and PLP mice. Scale bar: 200 μm. (G and H) Representative images (G) and quantification (H) of Iba1-positive microglial cells immunostaining in the striatum of control, CMVie/hSyn-mTFEB–injected, and MBP-mTFEB–injected WT and PLP mice. Scale bar: 50 μm. n = 5 per group. White bars, control; blue bars, CMVie/hSyn-mTFEB-HA; green bars, MBP-mTFEB-3×Flag. Data represent mean ± SEM. Comparisons were made using 2-way ANOVA and Tukey’s correction for multiple comparisons. *P < 0.05 compared with WT animals.