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. 2019 Aug 22;4(16):e125166. doi: 10.1172/jci.insight.125166

Figure 5. Links of EGFR expressions to gut barrier integrity.

Figure 5

(A and B) The EGFR expressions were correlated with genes associated with gut barrier integrity (CLDN3, TJP1, MUC2, LGR5, and SOX9) in intestines of patients with IBD (Sleiman’s, GEO ID GSE10616, n = 58; Vemeire’s, GEO ID GSE75214, n = 194; and Haberman’s GEO ID GSE57945, n = 194). Analysis of Vemeire’s samples was performed with colonic mRNA (B). All values were based on Pearson’s correlation analysis (R, Pearson’s correlation coefficient). (C–E) Six-week-old female mice were pretreated twice with vehicle, EcN, or EGF-EcN over 7 days (n = 12–15). The mice were then exposed to 3% DSS for 5 days to induce colitis. Claudin-3 (C), TJP1 (D), and cleaved caspase-3 (E) were detected and counterstained with hematoxylin (original magnification, ×200; inset magnification, ×800). Scale bar: 100 μm. A quantitative comparison is shown in each right graph. The asterisks represent significant differences between 2 groups (*P < 0.05; **P < 0.01; ***P < 0.001; ns using 2-tailed, unpaired Student’s t test).