Skip to main content
. 2019 Aug 22;4(16):e125437. doi: 10.1172/jci.insight.125437

Figure 3. Antiinflammatory activity of MSCs is mediated by integrin subtypes on macrophages, not by exosomes.

Figure 3

(A) hMSCs were pretreated with GW4869, a blocker of exosome production, before coculture with THP-1 macrophages. Gene expression of CXCL10 of macrophages was assessed after coculture with vehicle or GW4869-treated hMSCs. n = 4. (B) THP-1 macrophages were pretreated with cytochalasin D, an inhibitor of exosome uptake, before coculture with hMSCs. n = 4. (C) Coculture with hMSCs does not suppress inflammatory activation of macrophages pretreated with neutralizing antibody against integrin β1 or integrin αvβ3. Thus, integrin subtypes on THP-1 macrophages may be involved in the antiinflammatory action of ANGPTL4 released from hMSCs. n = 4. Data are represented as mean ± SEM. ##P < 0.01; ###P < 0.001 (by Student’s t test or 1-way ANOVA with Bonferroni’s multiple-comparisons test).