Figure 5. ANGPTL4 is essential for MSCs to suppress peritoneal macrophage activation.

(A) Experimental procedure with mouse peritonitis. Peritonitis was induced by thioglycollate injection for 3 days. CCM collected from wild-type mMSCs or knockout mMSCs was injected into the peritoneum followed by LPS stimulation for 4 hours. Peritoneal macrophages were isolated for further analyses. Circulating ANGPTL4 and IL-6 in plasma (B) and peritoneal lavage (C) were quantified. (D) Inflammation-related genes were assessed in peritoneal macrophages collected from mice with peritonitis (n = 8). Mice were injected with vehicle (Veh) or CCM i.p. on days 0 and 2. n = 5 for circulating ANGPTL4; n = 7 for circulating IL-6; n = 6 for ANGPTL4 and IL-6 in peritoneal lavage. Data are represented as mean ± SEM. ^#P < 0.05; ^##P < 0.01; ^###P < 0.001 (by Student’s t test or 1-way ANOVA with Bonferroni’s multiple-comparisons test).