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. Author manuscript; available in PMC: 2019 Oct 4.
Published in final edited form as: Adv Exp Med Biol. 2019;1164:119–139. doi: 10.1007/978-3-030-22254-3_10

Fig. 4. Knockdown of overexpressed splicing factors causes splice switching of PIK3CD variants.

Fig. 4

a) siRNA-mediated knockdown of three splicing factors in an AA cell line switches predominant expression of PIK3CD from the –S to the –L variant. Blots were quantified by densitometry and numbers underneath blots represent the -S/-L expression ratio. Shown are representative blots from 3-4 independent experiments. b) Western blot confirms knockdown of splicing factors at the protein level. Abbreviations: F2, SRSF2; F7, SRSF7; PF, hnRNPF; β, β-actin. Shown are representative blots from 3–4 independent experiments.