Skip to main content
. 2019 Sep 5;4(17):e129856. doi: 10.1172/jci.insight.129856

Figure 4. IFN-I triggers PGRN expression following viral infection.

Figure 4

(A) WT mice were infected with 2 × 106 pfu LCMV (WE strain). Organs were collected at day 1 after viral infection and subjected to qPCR analysis. The relative mRNA levels were normalized to Gapdh (n = 6). (B) Serum PGRN protein concentrations were determined by ELISA at indicated time points after LCMV infection (2 × 106 pfu, n = 3). (C) Clodronate treated (day –1) WT mice were infected with 2 × 106 pfu LCMV-WE. Serum PGRN levels (day 1) were measured by ELISA (n = 7). (D) WT and Ifnar1–/– BMDMs were infected with LCMV-WE (MOI = 1) for 48 hours. PGRN protein levels in cell supernatants were examined by ELISA (n = 6). (E and F) Ifnar1–/– and WT mice were infected with 2 × 106 pfu LCMV-WE. The Grn mRNA levels in the spleen were measured at day 1 following infection by qPCR (E, n = 3), and serum PGRN levels (F, n = 6) were determined by ELISA. Data in A–G show mean ± SEM. Each symbol represents an individual mouse. P values calculated by 2-way ANOVA (C–F) and Student’s t test (A and B); *P < 0.05; **P < 0.001; ***P < 0.001; ****P < 0.0001.