Skip to main content
. 2019 Sep 5;4(17):e130402. doi: 10.1172/jci.insight.130402

Figure 1. Demonstration of increased levels of 2PK channels in vagal sensory ganglia following 2-week HFD.

Figure 1

(A) RT-PCR data showing TRESK, TASK1 and -3, TREK2, and TRAAK2 mRNA in vagal sensory ganglia after 2 weeks of HFD feeding when compared with LFD-fed rats. HPRT was used as a loading control. n = 9 in each group. *P < 0.05 vs. LFD. Data are represented as mean ± SEM. One-way ANOVA with Bonferroni’s test. (B) A representative immunoblot from the vagal sensory ganglia shows TRESK protein expression after 2 weeks of HFD feeding (n = 5, and P < 0.05 compared with 2 weeks of LFD); GAPDH was used as a loading control. Summary histogram shows significant upregulation of TRESK protein expression in vagal sensory ganglia as early as 2 weeks after HFD feeding when compared with LFD-fed group. Student’s t test. *P < 0.05 vs. LFD. HFD, high-fat diet; HPRT, hypoxanthine-guanine phosphoribosyltransferase; LFD, low-fat diet; RT-PCR, reverse transcription PCR; TASK, TWIK-related acid-sensitive K+ channel; TREK2, TWIK-related K+ channel type 2; TRESK, TWIK-related spinal cord K+ channel.