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. 2019 Sep 5;4(17):e131310. doi: 10.1172/jci.insight.131310

Figure 2. JAK2L mice are lipodystrophic and have a defective fasted-to-fed response in adipose tissue.

Figure 2

(A) Body weight in grams (g) of ad lib–fed and 16-hour-fasted control CON, JAK2L, and JAK2LA mice. (B) Percentage of body weight lost following a 16-hour fast. (C) Percentages of lean and fast mass. Amount of (D) epididymal and (E) inguinal fat mass as a percentage of total body weight. (F) H&E staining of inguinal fat pads from 16-hour-fasted mice (original magnification, ×10). (G) Inguinal adipose levels (n = 3 per condition) of phosphorylated (T389) p70S6K (top) and total p70S6K (bottom) in 16-hour-fasted (–) or 30-minute-refed (+) mice as determined by Western blot. (H) Densitometric quantification of Western blots from (G) plotting phosphorylated T389/total p70S6K. n = 10–15 (A and B), 12–17 (C), and 5–7 (D and E). *P < 0.05; **P < 0.01; ****P < 0.0001 by 1-way (B and E) or 2-way (A, C, and H) ANOVA.