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. 2019 Sep 23;8:e47625. doi: 10.7554/eLife.47625

Figure 5. D cyclin inhibition prevents digit development caused by loss of Shh signalling.

Figure 5.

Application of DMEM (a) and PD0332991 at HH24 (b) does not affect digit patterning (n = 12/12 in both cases). Application of cyclopamine at HH20 results in a 1-2-2-2 digit pattern (c, f - n = 6/42, 14%), a 1-2-2-3 pattern (d, f - n = 15/42, 36%) and a 1-2-3 pattern (f, - n = 21/42, 50%) in day 10 wings. Application of cyclopamine at HH20 and PD0332991 at HH24 results in a 1-2-2 digit pattern (e, f, n = 9/23–40%), a 1-2-3 pattern (f - n = 12/23, 52%) a 1-2-2-2 pattern and 1-2-2-3 pattern (f – both n = 1/23, 4%) in day 10 wings. 50% of cyclopamine-treated wings have four digits, 8% of cyclopamine/PD033299-treated wings have four digits (g, χ2 test p =< 0.0001). Scale bars: 1 mm.