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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Eur J Neurosci. 2019 Jun 19;50(5):2801–2813. doi: 10.1111/ejn.14429

Table 1:

Details of viral injections and systemic injections

Animal
ID		 Species Injection
location		 Vector Titer
(vg/ml)		 Amount
injected,
(μl)		 Number
of
systemic
CNO or
CLZ
injections		 Survival
days
after
viral
injection		 Survival
days
after
last CNO
or CLZ
injection		 Days from
perfusion to
immunohisto-
chemistry
MR264 Rhesus macaque BLA, left# AAV5-hSyn-hM4Di-mCherrya 1.3×1013 40 9 651 73 180
MR263 Rhesus macaque STN, left AAV5-hSyn-hM4Di-mCherrya 4.1×1013 10 3 70 15 181
MR275 Rhesus macaque STN, left AAV5-hSyn-hM4Di-mCherrya 4.1×1013 12 27 417 143 62
STN, right AAV8-hSyn-hM4Di-mCherrya 2×1012 28 279
MR290 Rhesus macaque Dorsal amygdala, right AAV5-hSyn-HA-hM4Dic 6.9×1013 24 1* 36 7 139
Frontal cortex, right 15 50
MR293 Rhesus macaque Cortex area 25 hSyn-hM3Dq-mCherrya 3.4×1012 22 1* 41 14 96
RM8, RM9 Mice BLA, bilateral AAV8-hSyn-hM4Di-mCherryb 3×1012 1 0 9 n/a 42
RM34, RM35, RM36 Mice BLA,bilateral AAV5-hSyn-HA-hM4Dic 4.25×1012 0.3 (per side) 1 * 35 7 36

Viral vector sources:

a

University of North Carolina Vector Core,

b

Addgene,

c

Boston Children’s Hospital Viral Core

Abbreviations: CNO, clozapine-N-oxide; CLZ, clozapine

#

For monkey MR264, only the injection in the left BLA was examined, due to poor tissue preservation in the right hemisphere.

*

Only one injection of trace dose of [C11]-clozapine