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. Author manuscript; available in PMC: 2020 Oct 3.
Published in final edited form as: Mol Cell. 2019 Aug 14;76(1):177–190.e5. doi: 10.1016/j.molcel.2019.06.044

Figure 4. Auxin responsiveness corresponds with ARF nucleo-cytoplasmic Partitioning.

Figure 4

(A) Fluorescence microscopy imaged from 5d-old DR5-GFP (Ottenschläger et al., 2003; Sabatini et al., 1999), DII-Venus (Brunoud et al., 2012), and mII-Venus (Brunoud et al., 2012) ± 2-hour treatment of 10 µM IAA. Images were taken and quantified from upper root and root tip sections (+SD; n ≥ 20).

(B) Microscopy images of primary roots from 8d-old Col-0 (---; Wt), Col-0 carrying UBQ10:YFP-ARF19 (ARF19), and Col-0 carrying UBQ10:YFP-ARF19K962A (ARF19K) with the DR5:GUS reporter (Ulmasov et al., 1997) that were treated with a mock (Ethanol) or 10 µM IAA treatment for 2 hours prior to staining.