KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Chemicals, Peptides, and Recombinant Proteins | ||
| Propidium Iodide | Invitrogen | P21493 |
| Indole-3-Acetic Acid (IAA) | Sigma-Aldrich | I2886 |
| Basta | GoldBio | P-165–1 |
| DAPI | Invitrogen | D21490 |
| HA-Tag (C29F4) Rabbit mAb | Cell Signaling Technologies | 3724 |
| Critical Commercial Assays | ||
| RNeasy Plant Mini Kit | Qiagen | 74904 |
| Ribo-Zero Gold Kit | Epicentre | N/A |
| QuikChange Lightning Multi Site-Directed Mutagenesis Kit | Agilent | 210518 |
| XT Codeset Gene Expression Assay | Nanostrings | |
| Deposited Data | ||
| RNASeq data | This study | GEO: GSE104385 |
| Experimental Models: Organisms/Strains | ||
| Arabidopsis: pUBQ10:YFP-ARF19 | This study | N/A |
| Arabidopsis: pUBQ10:YFP-ARF19K962A | This study | N/A |
| Arabidopsis: pUBQ10:YFP-ARF19PB1 | This study | N/A |
| Arabidopsis: pUBQ10:YFP-ARF19MR+PB1 | This study | N/A |
| Arabidopsis: p35S:ARF7-HA | Cho et al., 2014 | N/A |
| Arabidopsis: p35S:ARF7-GFP | Okushima et al., 2007 | N/A |
| Arabidopsis: pARF7:gARF7-YFP | This study | N/A |
| Arabidopsis: pUBQ10:YFP-ARF2 | This study | N/A |
| Arabidopsis: DR5-GFP | Sabatini et al., 1999 | N/A |
| Arabidopsis: DR5-GUS | Ulmasov et al., 1997 | N/A |
| Arabidopsis: DII-Venus | Brunoud et al., 2012 | N/A |
| Arabidopsis: mII-Venus | Brunoud et al., 2012 | N/A |
| Oligonucleotides | ||
| Primers used in this study, see Method Details | This study | N/A |
| Recombinant DNA | ||
| pUBQ10:YFP-GW | Michniewiczet al., 2015 | ABRC stock #CD3–1948 |
| cDNA C105352 (ARF19) | Arabidopsis Biological Resource Center (ABRC) | AY669794 |
| cDNA U12525 (ARF2) | Arabidopsis Biological Resource Center (ABRC) | BT001072 |
| cDNA U79557 (ARF5) | Arabidopsis Biological Resource Center (ABRC) | 2708483 |
| Software and Algorithms | ||
| ImageJ | NIH | https://imagej.nih.gov/ij/ |
| PLAAC algorithm | Lancaster et al., 2014 | http://plaac.wi.mit.edu |
| IUPred algorithm | Dosztányi et al., 2005 | http://iupred.enzim.hu |
| CAMPARI Monte Carlo simulation engine | Vitalis & Pappu, 2009 | http://campari.sourceforge.net) |
| MDTraj and CTraj | McGibbon et al., 2015 | http://pappulab.wustl.edu/CTraj.html |
| Spliced Transcripts Alignment to a Reference (STAR) | Dobin et al., 2012 | http://code.google.com/p/rna-star/ |
| RSeQC | Wang & Wang, 2012 | http://code.google.com/p/rseqc |
| R/Bioconductor | Gentleman et al., 2004 | https://www.bioconductor.org |
| nSolver Analysis software | Nanostrings | https://www.nanostring.com/products/analysis-software/nsolver |
| SimFCS | Laboratory for Fluorescence Dynamics | https://www.lfd.uci.edu/globals/ |
| Other | ||