A. H&E staining of livers showed that dietary fat increased steatosis. No noticeable effect of DG-041 treatment on steatosis was observed. In VHFD mice treated with DG-041, hepatocellular ballooning was observed. B. Liver ORO staining was increased with increasing dietary fat. DG-041 appeared to increase ORO staining only in mice fed the VHFD. Images are a representative sample of sections from 4 control + vehicle, 3 control + DG-041, 3 HFD + vehicle, 4 HFD + DG-041, 4 VHFD + vehicle, 5 VHFD + DG-041.