Table 2. Fatty acid composition of skeletal muscle triglycerides.
Fatty acid composition of skeletal muscle triglycerides from male EP3+/+ and EP3−/− mice, fed either HFD or control diet. N = 4 control + vehicle, 3 control + DG-041, 4 HFD + vehicle, 4 HFD + DG-041, 4 VHFD + vehicle, 5 VHFD + DG-041.
| Parameter | control + vehicle | control + DG-041 | HFD + vehicle | HFD + DG-041 | VHFD + vehicle | VHFD + DG-041 | ANOVA p-value | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| g/100 g fatty acids | ||||||||||||||||
| 14:0 | 1.63 | 0.08 | 1.57 | 0.14 | 0.97 | 0.07 | 1.00 | 0.03 | 0.74 | 0.03 | 0.91 | 0.04 | 0.113 | < 0.001 | 0.015 | |
| 16:0 | 16.86 | 0.78 | 17.94 | 0.79 | 17.24 | 0.85 | 17.15 | 0.59 | 17.11 | 0.41 | 18.24 | 0.98 | 0.038 | 0.453 | 0.228 | |
| 16:1 | 18.53 | 0.84 | 17.60 | 2.01 | 7.15 | 0.53 | 7.48 | 0.90 | 4.88 | 0.49 | 5.93 | 0.44 | 0.692 | < 0.001 | 0.127 | |
| 17:0 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | NA | NA | NA | |
| 18:0 | 1.18 | 0.23 | 1.27 | 0.24 | 2.58 | 0.23 | 2.62 | 0.36 | 3.24 | 0.10 | 3.02 | 0.18 | 0.737 | < 0.001 | 0.382 | |
| 18:1ω9 | 42.05 | 0.59 | 41.99 | 1.81 | 47.25 | 0.92 | 47.33 | 0.28 | 50.00* | 0.54 | 46.78* | 0.73 | 0.008 | < 0.001 | 0.001 | |
| 18:1ω7 | 4.42 | 0.26 | 4.44 | 0.18 | 1.88 | 0.22 | 2.12 | 0.21 | 1.46** | 0.15 | 2.29** | 0.33 | 0.002 | < 0.001 | 0.010 | |
| 18:2 | 14.34 | 0.49 | 14.00 | 1.40 | 21.41 | 0.59 | 20.55 | 0.46 | 21.63 | 0.34 | 21.55 | 0.31 | 0.116 | < 0.001 | 0.455 | |
| 18:3ω6 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | NA | NA | NA | |
| 18:3ω3 | 0.71 | 0.48 | 0.82 | 0.07 | 0.85 | 0.06 | 0.88 | 0.05 | 0.66 | 0.03 | 0.82 | 0.05 | 0.235 | 0.446 | 0.781 | |
| 20:3ω6 | 0.00 | 0.00 | 0.00 | 0.00 | 0.13 | 0.09 | 0.19 | 0.01 | 0.11 | 0.01 | 0.03 | 0.08 | 0.720 | < 0.001 | 0.062 | |
| 20:4 | 0.28 | 0.32 | 0.37 | 0.32 | 0.41 | 0.10 | 0.48 | 0.05 | 0.18 | 0.03 | 0.39 | 0.06 | 0.110 | 0.206 | 0.695 | |
| 20:5 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | NA | NA | NA | |
| 22:4ω6 | 0.00 | 0.00 | 0.00 | 0.00 | 0.03 | 0.05 | 0.03 | 0.06 | 0.00 | 0.00 | 0.00 | 0.00 | 0.948 | 0.166 | 0.948 | |
| 22:5ω6 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | NA | NA | NA | |
| 22:5ω3 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | NA | NA | NA | |
| 22:6 | 0.00 | 0.00 | 0.00 | 0.00 | 0.13 | 0.09 | 0.19 | 0.02 | 0.02 | 0.03 | 0.03 | 0.06 | 0.240 | < 0.001 | 0.458 | |
| Ratio | ||||||||||||||||
| 16:0/16:1 | 0.91 | 0.08 | 1.03 | 0.08 | 2.43 | 0.24 | 2.33 | 0.38 | 3.54 | 0.49 | 3.10 | 0.39 | 0.310 | < 0.001 | 0.255 | |
| 18:0/18:1 | 0.03 | 0.00 | 0.03 | 0.00 | 0.05 | 0.01 | 0.05 | 0.01 | 0.06 | 0.00 | 0.06 | 0.00 | 0.908 | < 0.001 | 0.822 | |
| Saturated / Unsaturated† | 0.24 | 0.01 | 0.26 | 0.01 | 0.26 | 0.02 | 0.26 | 0.01 | 0.27 | 0.01 | 0.29 | 0.02 | 0.071 | 0.022 | 0.386 | |
†
(14:0+16:0+17:0+18:0) / (16:1+18:1ω9+18:1ω7+18:2+18:3ω6+18:3ω3+20:3ω6+20:4+20:5+22:4ω6+22:5ω6+22:5ω3+22:6)
*
Bonferroni post-hoc comparison P<0.05
**
Bonferroni post-hoc comparison P<0.01
***
Bonferroni post-hoc comparison P<0.001