KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse anti-Nestin | Abcam | ab22035 |
| Rat anti-CTIP2 | Abcam | ab18465 |
| Rabbit anti-SATB2 | Abcam | ab34735 |
| Chicken anti-MAP2 | Abcam | ab5392 |
| Rabbit anti-Ki67 | Abcam | ab15580 |
| Rabbit anti-TBR1 | Abcam | ab31940 |
| Rabbit anti-TBR2 | Abcam | ab23345 |
| Rabbit anti-beta-catenin | Abcam | E247 |
| Mouse anti-GABA | Abcam | ab86186 |
| Mouse anti-GABA B Receptor 1 | Abcam | ab55051 |
| Rabbit anti-Calretinin | Abcam | ab92341 |
| Rabbit anti-TTF1 (NKX2.1) | Abcam | ab76013 |
| Rabbit anti-Synapsin1 | Millipore | AB1543P |
| Mouse anti-NeuN | Millipore | MAB377 |
| Mouse anti-Parvalbumin | Millipore | MAB1572 |
| Rat anti-Somatostatin | Millipore | MAB354 |
| Rabbit anti-SOX2 | Cell Signaling | 2748 |
| Rabbit anti-GFAP | DAKO | Z033429 |
| Donkey anti-Mouse IgG- Alexa Fluor 488 | Thermo Fisher | R37114 |
| Donkey anti-Rabbit IgG- Alexa Fluor 488 | Thermo Fisher | R37118 |
| Donkey anti-Rat IgG- Alexa Fluor 488 | Thermo Fisher | A21208 |
| Goat anti-Mouse IgM- Alexa Fluor 488 | Thermo Fisher | A21042 |
| Goat anti-Chicken IgY- Alexa Fluor 488 | Thermo Fisher | A11039 |
| Donkey anti-Rabbit IgG- Alexa Fluor 555 | Thermo Fisher | A31572 |
| Donkey anti-Mouse IgG- Alexa Fluor 555 | Thermo Fisher | A31570 |
| Donkey anti-Mouse IgG- Alexa Fluor 647 | Thermo Fisher | A31571 |
| Donkey anti-Rabbit IgG- Alexa Fluor 647 | Thermo Fisher | A31573 |
| Goat anti-Mouse IgM- Alexa Fluor 647 | Thermo Fisher | A21238 |
| Goat anti-Chicken IgY- Alexa Fluor 647 | Thermo Fisher | A21449 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Dorsomorphin | R&D Systems | 3093 |
| Stemolecule SB431542 | StemGent | 04-0010-10 |
| ROCK inhibitor (Ri) Y-27632 dihydrochloride | Sigma-Aldrich | 688000 |
| FGF-Basic (AA 1-155) Recombinant Human Protein | Life Technologies | PHG0263 |
| Animal-Free Recombinant Human EGF | Peprotech | AF-100-15 |
| Recombinant Human BDNF | Peprotech | 450-02 |
| Recombinant Human GDNF | Peprotech | 450-10 |
| Recombinant Human NT-3 | Peprotech | 450-03 |
| L-Ascorbic acid | Sigma-Aldrich | A4403 |
| N6,2′-O-Dibutyryladenosine 3′,5′-cyclic monophosphate sodium salt | Sigma-Aldrich | D0627 |
| Bicuculline methiodide | Tocris | 2503 |
| Picrotoxin | Tocris | 1128 |
| Muscimol | Tocris | 0289/1 |
| CNQX disodium salt | Tocris | 1045 |
| D-AP5 | Tocris | 0106 |
| (R)-Baclofen | Tocris | 0796/10 |
| Tetrodotoxin citrate | Tocris | 1069 |
| Critical Commercial Assays | ||
| Chromium Single Cell 3’ v2 Library kit | 10X Genomics | PN-120237 |
| MyOne Silane Beads | Thermo Fisher | 37002D |
| SPRIselect Reagent Kit | Beckman Coulter | B23317 |
| Qubit dsDNA HS Assay Kit | Thermo Fisher | Q32854 |
| Deposited Data | ||
| Single-cell RNA sequencing data | This paper | NCBI GEO: GSE130238 |
| Experimental Models: Cell Lines | ||
| Control iPSCs | N/A | N/A |
| Experimental Models: Organisms/Strains | ||
| C57 black 6 | The Jackson Laboratory | C57BL6 |
| Software and Algorithms | ||
| GraphPad Prism | GraphPad Software | N/A |
| AxIS Software | Axion Biosystems | N/A |
| Neural Metrics Tool | Axion Biosystems | N/A |
| MATLAB | MathWorks | N/A |
| Cell Ranger software version 2.1.1 | 10X Genomics | N/A |