Antibodies |
Mouse anti-Nestin |
Abcam |
ab22035 |
Rat anti-CTIP2 |
Abcam |
ab18465 |
Rabbit anti-SATB2 |
Abcam |
ab34735 |
Chicken anti-MAP2 |
Abcam |
ab5392 |
Rabbit anti-Ki67 |
Abcam |
ab15580 |
Rabbit anti-TBR1 |
Abcam |
ab31940 |
Rabbit anti-TBR2 |
Abcam |
ab23345 |
Rabbit anti-beta-catenin |
Abcam |
E247 |
Mouse anti-GABA |
Abcam |
ab86186 |
Mouse anti-GABA B Receptor 1 |
Abcam |
ab55051 |
Rabbit anti-Calretinin |
Abcam |
ab92341 |
Rabbit anti-TTF1 (NKX2.1) |
Abcam |
ab76013 |
Rabbit anti-Synapsin1 |
Millipore |
AB1543P |
Mouse anti-NeuN |
Millipore |
MAB377 |
Mouse anti-Parvalbumin |
Millipore |
MAB1572 |
Rat anti-Somatostatin |
Millipore |
MAB354 |
Rabbit anti-SOX2 |
Cell Signaling |
2748 |
Rabbit anti-GFAP |
DAKO |
Z033429 |
Donkey anti-Mouse IgG- Alexa Fluor 488 |
Thermo Fisher |
R37114 |
Donkey anti-Rabbit IgG- Alexa Fluor 488 |
Thermo Fisher |
R37118 |
Donkey anti-Rat IgG- Alexa Fluor 488 |
Thermo Fisher |
A21208 |
Goat anti-Mouse IgM- Alexa Fluor 488 |
Thermo Fisher |
A21042 |
Goat anti-Chicken IgY- Alexa Fluor 488 |
Thermo Fisher |
A11039 |
Donkey anti-Rabbit IgG- Alexa Fluor 555 |
Thermo Fisher |
A31572 |
Donkey anti-Mouse IgG- Alexa Fluor 555 |
Thermo Fisher |
A31570 |
Donkey anti-Mouse IgG- Alexa Fluor 647 |
Thermo Fisher |
A31571 |
Donkey anti-Rabbit IgG- Alexa Fluor 647 |
Thermo Fisher |
A31573 |
Goat anti-Mouse IgM- Alexa Fluor 647 |
Thermo Fisher |
A21238 |
Goat anti-Chicken IgY- Alexa Fluor 647 |
Thermo Fisher |
A21449 |
Chemicals, Peptides, and Recombinant Proteins |
Dorsomorphin |
R&D Systems |
3093 |
Stemolecule SB431542 |
StemGent |
04-0010-10 |
ROCK inhibitor (Ri) Y-27632 dihydrochloride |
Sigma-Aldrich |
688000 |
FGF-Basic (AA 1-155) Recombinant Human Protein |
Life Technologies |
PHG0263 |
Animal-Free Recombinant Human EGF |
Peprotech |
AF-100-15 |
Recombinant Human BDNF |
Peprotech |
450-02 |
Recombinant Human GDNF |
Peprotech |
450-10 |
Recombinant Human NT-3 |
Peprotech |
450-03 |
L-Ascorbic acid |
Sigma-Aldrich |
A4403 |
N6,2′-O-Dibutyryladenosine 3′,5′-cyclic monophosphate sodium salt |
Sigma-Aldrich |
D0627 |
Bicuculline methiodide |
Tocris |
2503 |
Picrotoxin |
Tocris |
1128 |
Muscimol |
Tocris |
0289/1 |
CNQX disodium salt |
Tocris |
1045 |
D-AP5 |
Tocris |
0106 |
(R)-Baclofen |
Tocris |
0796/10 |
Tetrodotoxin citrate |
Tocris |
1069 |
Critical Commercial Assays |
Chromium Single Cell 3’ v2 Library kit |
10X Genomics |
PN-120237 |
MyOne Silane Beads |
Thermo Fisher |
37002D |
SPRIselect Reagent Kit |
Beckman Coulter |
B23317 |
Qubit dsDNA HS Assay Kit |
Thermo Fisher |
Q32854 |
Deposited Data |
Single-cell RNA sequencing data |
This paper |
NCBI GEO: GSE130238
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|
|
|
Experimental Models: Cell Lines |
Control iPSCs |
N/A |
N/A |
|
|
|
Experimental Models: Organisms/Strains |
C57 black 6 |
The Jackson Laboratory |
C57BL6 |
|
|
|
Software and Algorithms |
GraphPad Prism |
GraphPad Software |
N/A |
AxIS Software |
Axion Biosystems |
N/A |
Neural Metrics Tool |
Axion Biosystems |
N/A |
MATLAB |
MathWorks |
N/A |
Cell Ranger software version 2.1.1 |
10X Genomics |
N/A |