Figure 8.

Developmental and growth parameters of embryos cultured in 100% rat serum (RS) or in serum diluted 30:70 with GMEM plus DS. (a) Yolk sac diameter shows no difference between 100% and diluted RS cultures, whereas dorsal length and head length are both significantly diminished in the 30% RS cultures. (b) Somite number does not differ significantly in embryos cultured in 100% or 30% RS. (c) Total protein content shows a significant reduction in 30% RS compared with 100% RS controls. Statistical analysis: **p ≤ .01, *p ≤ .05; Student's t tests. RS: n = 7, 30% RS: n = 11