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. 2019 Oct 4;9:14324. doi: 10.1038/s41598-019-50550-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Inflammatory response throughout the disease progression. (a) Inflammation was assessed in rectus femoris using CD11b antibody against leukocytes (red). DAPI was used to visualise nuclei. Infiltrates of inflammatory cells become evident at day 4, are highly pronounced at day 7, and decrease afterwards. (b) Quantification of CD11b stained areas in wild-type and dy^3K/dy^3K muscle. There is a clear trend for increased inflammation in 4- and 7-day-old dystrophic muscle, and the CD11b-positive areas are significantly larger in 14- and 21-day-old dy^3K/dy^3K muscle (p = 0.0004 and p = 0.0126, respectively) compared to age-matched wild-type muscle Scale bar: 40 µm.