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. 2019 Oct 4;9:14314. doi: 10.1038/s41598-019-50043-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Uptake of nanoparticles in macrophages studied by flow cytrometry. (a) Histogram represents the uptake of NP/Coumarin-6 and Folate-NP/Coumarin-6 by Raw 264.7 macrophages with (+) LPS stimulation (1 µg/ml) in the presence (+) or absence (−) of folic acid (FA) (a competitor). (b) The mean fluorescent intensity of cells from histogram. The data are shown as the mean ± standard deviation (n = 4), and 10,000 cells were counted. *P < 0.05.