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. 2019 Oct 4;9:14328. doi: 10.1038/s41598-019-50908-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Clonogenic assay and cell survival at 220 kV and 4 MV. (A) left panel: Survival fraction (SF) of HUVECs irradiated at 220 kV (orange curve) and 4 MV (blue curve). Right panel: the associated RBE curve defined as a ratio of doses for a given SF (solid line) and its associated bootstrap confidence intervals (dotted lines). The green arrow representing the range of SF wherein the value of RBE is significantly different from 1. (B) Representations of 3D and 2D curves of cell survival measures with trypan blue counting method for 220 kV and 4 MV. (C) Representations of 3D and 2D curves of cell viability ratio [220 kV/4 MV].