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. 2019 Oct 4;9:14328. doi: 10.1038/s41598-019-50908-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cell Division (CellTrace Technology)/ Senescence (C₁₂FDG) (Flow Cytometry) bi-parametric analysis (A) Example of flow cytometry measurements obtained at D7 for control and 6 Gy irradiation at 4 MV. (B) Percentage of β-GAL-positive cells (Q1 + Q2) compared to control at 4 MV (blue dots) and 220 kV (orange dots) irradiations. Each dot represents one independent experiment (****p < 0.001, one-way Anova). (C) Percentage of divided cells (Q1 + Q3) compared to control for 220 kV (orange dots) and 4 MV (blue dots). Each dot represents one independent experiment (***p < 0.001, ****p < 0.001, one-way Anova).