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. 2019 Oct 4;10:4523. doi: 10.1038/s41467-019-12433-w

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Fig. 5 — PP2A regulates podocyte actin cytoskeleton and motility through DBN1 regulation. a Western blot of DBN1 in diabetic and control eNOS^−/− mouse glomeruli treated with vehicle or ATG. b Western blot of DBN in podocytes cultured under normal or high glucose conditions with ATG and/or okadaic acid (OA) treatments as indicated. c Phos-tag gel and western blot analysis of immortalized podocytes stably transfected with either empty vector (EV) or expression vector expressing either wildtype (WT) DBN1, DBN1 T335A (top), or DBN1 S337A (bottom). d Lysates of podocytes stably expressing FLAG-tagged DBN1 (WT or T335A) were immunoprecipitated with anti-FLAG antibody and immunoblotted for F-actin. The top panels show the western blot total input and the bottom panel shows the immunoprecipitated proteins. e Adhesion assay in podocytes stably expressing DBN1 T335A in comparison with those with empty vector (EV) or DBN1 WT expression, n = 3. f Migration assay of podocytes stably expressing DBN1 T335A in comparison with those with empty vector (EV) or DBN1 WT expression, n = 7. **P < 0.01 or ***P < 0.001 compared to all other groups by one-way ANOVA with Tukey’s post hoc analysis. The data are represented as mean ± SD. Source data are provided as a Source Data file