Table 1.

Baits used in the AP-MS experiments for the characterization of the midbody interactome

Name	Function	Localization	Tag and cell line reference
Anillin	Actomyosin binding protein; contractile ring scaffolding	Cleavage furrow, midbody ring and secondary constriction sites	GFP48
Aurora B	Serine/threonine kinase; CPC component, controls furrow ingression, central spindle formation and abscission	Cleavage furrow, central spindle, midbody arms	GFP (this study)
CHMP4B	ESCRT-III protein; required for abscission	Midbody core and abscission site	GFP49
CHMP4C	ESCRT-III protein; required for abscission	Central spindle, midbody arms, midbody core and abscission site	Flag26
Citron kinase (CIT-K)	Serine/threonine kinase; required for midbody assembly, organization and maturation	Cleavage furrow, central spindle, midbody ring	AcGFP11
Ect2	Rho GEF; activates RhoA to promote contractile ring assembly and constriction	Cleavage furrow, central spindle, midbody core	AcGFP50
KIF14	Kinesin, CIT-K partner; required for midbody assembly, organization and maturation	Cleavage furrow, central spindle, midbody ring	GFP51
KIF20A/MKLP2	Kinesin; required for central spindle formation and CPC translocation	Central spindle, midbody arms	GFP51
KIF23/MKLP1	Kinesin; centralspindlin component, required for furrow ingression, central spindle and midbody formation	Central spindle, midbody core	GFP52
PRC1	Microtubule associated protein, required for central spindle and midbody formation	Central spindle, internal midbody arms and core	GFP53