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. 2019 Oct 4;9:14352. doi: 10.1038/s41598-019-50764-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The dependence of the initial rates of Abz-RSVIK(Dnp) hydrolysis by protealysin and thermolysin on the substrate concentration. The enzymes concentration was 2.5 nM. Values are represented as mean ± SD of two independent experiments with triplicates. Solid lines, fit of the data to the Michaelis-Menten equation (R² = 0.9939 for protealysin and R² = 0.9755 for thermolysin).