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Atp6ap2-deficient β cells have reduced insulin content. (A) Islet immunofluorescence for insulin (red) and glucagon (green) of mice after 3 and 8 wk of TAM treatment. Nuclear stain (DAPI) is shown in blue. (B and C) Quantification of A. n = 4 to 6. (D) Islet immunofluorescence for pancreatic and duodenal homeobox 1 (Pdx1; green) and insulin (red) from mice treated with TAM for 3 and 8 wk. (E) Quantification of D. n = 4 to 6. *P < 0.05, **P < 0.01. Scale bars are indicated.
