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. 2019 Sep 16;116(40):20226–20231. doi: 10.1073/pnas.1907379116

Fig. 4.

Fig. 4.

Wound-induced up-regulation of JAZ10, accumulation of jasmonic acid (JA), jasmonoyl-isoleucine (JA-Ile), oxo-phytodienoic acid (OPDA), and reduced insect growth in the aha1-7 mutant. (A) JAZ10 transcript levels in leaves 8 and 13 1 h after wounding (n = 4), (B) JA, (C) JA-Ile, and (D) OPDA in connected leaf 13, 45 min after wounding (n = 4). Cognate internal standards were used for JA and JA-Ile quantification. OPDA levels were assessed with the internal standard for JA-Ile. (E) Weight of S. littoralis larvae grown for 12 d on the WT and/or on the aha1-7 mutant. Each replicate consisted of 11 plants and 44 larvae. Data shown are combined from two replicated bioassays. Data shown are means ± SD. Asterisks refer to data significantly different from wounded WT: Student t test: *P < 0.05, **P < 0.01. For statistics in B–D, only data above the limit of quantification (LOQ) (dashed line) were considered as relevant. U, unwounded.