Fig. 2.

PI(4,5)P₂ effect is selective and moderately high affinity. (A–C) Representative current traces at +100 mV with 270 nM Ca²⁺ in the absence (black) and presence (red) of 10 µM (A) diC8-PI(4,5)P₂, (B) diC8-PI(3,4,5)P₃, and (C) diC8-PI(3,5)P₂. (D–F) Average ANO1 current–voltage relationships in the absence of phosphoinositides (black squares) and in the presence of 10 µM (D) diC8-PI(4,5)P₂, (E) diC8-PI(3,4,5)P₃, and (F) diC8-PI(3,5)P₂. (G) Summary of stimulatory effect of phosphoinositides on ANO1 current at 100 mV with 270 nM Ca²⁺. (H and I) Concentration–response curve for diC8-PI(4,5)P₂ on ANO1 current with 270 nM Ca²⁺at (H) 100 mV and (I) −100 mV.