Fig. 7.
The CD8 T cell clock controls a day–night difference in the control of an infectious challenge following DC-OVA vaccination. (A) Experimental protocol. BMDCs loaded with OVA peptide were injected i.v. at either CT6 or CT18 in E8I-Cre+/− Bmal1f/f (Δ/Δ) and their littermates without Cre (f/f). Seven days postvaccination, mice were challenged with 2 × 105 CFU of L. monocytogenes-OVA i.v. at ZT8. (B) Livers and (C) spleens were harvested to assess bacterial load. Data are pooled from 3 independent experiments with similar results, n = 10 to 13 mice/group. Two-way ANOVA with Bonferroni test where applicable. *P < 0.05, **P < 0.01, ***P < 0.001. See SI Appendix, Statistical Details for more information on statistics.