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. 2019 Oct 4;9:14344. doi: 10.1038/s41598-019-50696-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phylogenetic tree of the WRKY proteins of pigeonpea (Cajanus cajan). The conserved WRKY domain sequences of pigeonpea WRKY proteins were used to construct multiple sequence alignments using Clustal W. Software MEGA was used to prepare the phylogenetic tree and neighbor-joining method was adopted by 1,000 bootstrap replications. The CcWRKY proteins of pigeonpea are classified into three groups, Group I–III, based on conserved WRKY domain sequences. Yellow colour indicates the Arabidopsis WRKY defense responsive genes used for selection of pigeonpea WRKY defense responsive genes.