FIGURE 4.

Cytokine profiling of HMDMs and MDDCs exposed to NETs and/or LPS. Profiling of HMDMs (A) and MDDCs (B) exposed to LPS (100 ng/ml), purified NETs, LPS plus NETs, or control RPMI medium for 1 h, and then to the relevant culture media for 24 h. After 24 h, supernatants were collected and used for quantification with the Luminex platform. Data are displayed as average values of three independent experiments with cells from three different blood donors ± SEM. One-way ANOVA with Tukey posttest for comparison of all groups was carried out to evaluate statistical significance (where not specified, the differences between groups were NS). Refer to Supplemental Fig. 3 for additional results. *p < 0.05, **p < 0.01, ***p < 0.001.