Supplementary Table 1.

Primers for amplification of NOTCH3 exons.

Exons	Primer sequence (from 5′ to 3′)	Target (bp)	Annealing temperature (°C)
Exon 2	AGACCTCCCTGTAACACTCAGCACC	616	55
	AGAGCCCCTGCTCAGTTTCCAAT
Exon 3	GCAAATGTGTGTTTGCTGCT	482	55
	CACTCATCCACGTCGCTTC
Exon 4	TCCTAAACTCACCCTGTCCTG	559	60
	TTATAGGTGTTGACGCCATCC
Exon 5	GGCGACCTCACTTACGACTG	454	56
	GTCCAGCCATTGACACACAC
Exon 6	CTGGACTGCTGCATCTGTGT	283	55
	TGCAGAGAAAACGGCCACTC
Exon 7	TACTCAAGGGGTGTGGGC	319	55
	GATGGAGTGCGATCGGTGT
Exon 8	TGTGGACGAGTGCTCTATCG	400	57
	CCCACTTACACCCCATTCTG
Exon 9	GAGTGTAGAGGTGGGGACGA	407	55
	TGGGTGGAAAAACCCATTTA
Exon 10	CAGGGTGGGAACCTGTAAAA	481	56
	ACTTTGGCTCCACACGTAGC
Exon 11	GAGCTGAACCAGGATTGGTC	453	60
	TTCACTTCGCAGTTCACACC
Exon 12	AAATGCCTAGACCTGGTGGA	385	55
	CGTTGGACAAGAGTCTGCAA
Exon 13	GGTGTGCTAAGTGGGGTCAC	485	57
	CAGTGGAAACCCATTCCATC
Exon 14	AGGGAGGTTCCTGTGTGGAT	499	56
	AGCTCCTGGAGGGAAATGAT
Exon 15	CTCTCTCCTCCCGCTATGTT	578	58
	GCCGTCTCCCTGTCTCAGTA
Exon 16	TGACAGCACGGCTTATTTTG	476	55
	CCAGCTCATTCCCTAACTCG
Exon 17	CTGTCCTTGTCCTGTTCCAA	659	60
	CAGTCATCAGAGTCCGCAGT
Exon 18	CTTGCCGAGATAAGGGTCAG	450	55
	AGGGGAAGCACTCAGAGTCA
Exon 19	GGCACAGTTTCTCCCAGACTA	455	51
	CCTTCAACGCTCACAGCAG
Exon 20	GACTCATTCCACCAAGGATGTT	430	51
	CACCCACAGATACACCAAGAGT
Exon 21–23	ACCTACTGGTAGTGCGCTGAACATC	937	55
	TGTCAGCATTTTCCAGAAACTCCCT
Exon 24	GGAGCGGTTGGGTGGGACA	982	55
	GGCAGACAAACGGGGCAGAG