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. 2018 Jul 11;69(20):4757–4771. doi: 10.1093/jxb/ery239

Fig. 3.

Fig. 3.

Floral organ identity genes are misexpressed in the hda19 inflorescence meristem and are direct targets of HDA19 and TOPLESS. (A–L) RNA in situ hybridizations of AP3 (A–D), PI (E–H), and AG (I–L) in hda19 inflorescence apices. Numbers in the upper right indicate the inflorescence meristem (IM) phase. Arrowheads denote ectopic expression of floral organ identity genes in IMs. Stamenoid organs (arrows) of phase 4 IMs show strong expression of floral organ identity genes. Scale bar: 50 µm. (M) Anti-GFP ChIP showing specific binding of HDA19 and the co-repressor TOPLESS (TPL) to the promoter of AP3 and to the promoter and second intron of AG. A control ChIP was performed on non-transgenic wild-type (wt) tissue. Data were normalized relative to input and ACT2 abundance. Data are represented as the mean ±SE of at least two biological replicates. Student’s t-test was used to determine the significance of target enrichment relative to wt IP (*P<0.05; **P<0.01).