Table 2.

Amounts of various proteins deposited in cell matrix or released into pooled media by healthy and AAA human aortic SMCs, per cell, over a 21-day period, within 2D or 3D cultures, in the presence or absence of 100 nM GSNO. Data shown here represents the average of at least n = 6/ case. Data for the healthy Hu-SMCs were from our previous publication [22].

		GSNO dose (nM)	Fold-increase in proliferation, compared to initial seeding	Total protein amounts (μg)		Elastin (ng)		sGAGs (ng)		HA (pg)		LOX functional activity (pM)
				Cell matrix	Pooled media	Cell matrix	Pooled media	Cell matrix	Pooled media	Cell matrix	Pooled media	Cell matrix	Pooled media
3D cultures	Healthy Hu-SMCs	0	3.2	0.13	2.02	ND	7.06	ND	16.7	0.21	2.07	69	70
		100	2.03	0.12	3.12	1.31	29.9	0.49	7.08	0.5	3.2	370	1610
3D cultures	Hu-AAA- SMCs	0	2.05	0.27	37.9	< 0.01	0.17	< 0.01	0.036	0.57	27.6	0.1	2.76
		100	1.3	0.83	52.7	< 0.01	0.26	< 0.01	0.063	0.77	40.7	0.27	3.1
2D cultures	Hu-AAA- SMCs	0	2.8	0.016	16.8	< 0.01	0.15	< 0.01	0.015	0.36	22.8	0.02	0.18
		100	2.1	0.042	34.6	< 0.01	0.177	< 0.01	0.034	0.51	29.7	0.03	2.5

ND: Not detectable within assay limits (i.e., < 1 pg/cell amounts).