Fig. 2.

Inducing ADSCs to differentiate into IM cells. A Histology examination of ADSCs and IM cells: Uninduced ADSCs at 0 days (Ai); Uninduced ADSCs at 7 days (Aii); IM cells (Aiii). B Immunofluorescence for OSR1: in ADSCs at 0 days (Bi); ADSCs cultured at 7 days (Bii); and IM cells (Biii). C Immunofluorescence of PAX2: in ADSCs at 0 days (Ci); ADSCs cultured at 7 days (Cii); and IM cells (Ciii). The scale bar is 100 µm. D Western blotting for the expression of PAX2 and OSR1 in ADSCs and IM cells. Mouse embryos at day 9.5 (E9.5) were used as a positive control. PAX2 and OSR1 were not expressed in ADSCs but were highly expressed in E9.5 and IM cells induced from ADSCs