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. 2019 Aug 14;110(10):3275–3287. doi: 10.1111/cas.14154

Figure 2.

Figure 2

OSSL_325096 induces apoptosis in multiple myeloma (MM) cell lines. Cells were treated with DMSO or 1 μmol/L OSSL_325096 for 48 h. A, Dot plots and B, bar charts of cells stained with annexin V and 7AAD after 48‐h treatment. Pre‐apoptotic cells are defined as annexin V+ 7AAD − cells and dead cells are annexin V+ 7AAD + cells. Data represent the mean ± SD derived from three separate experiments. Significance values are indicated as ***P < .001 (Student's t‐test). C, Western blots of ubiquitin, non‐cleaved and cleaved caspase‐3, and actin at various time points in KMS12PE and KMS11 cells treated with OSSL_325096. Poly‐ubiquitinated proteins accumulated and apoptosis was induced in KMS12PE and KMS11 cells treated with 1 μmol/L OSSL_325096. D, Western blots of PERK, CHOP, IRE1α, ATF4, ATF6, XBP1 and XBP, and actin in OSSL_325096‐treated KMS12PE and KMS11 cells. Endoplasmic reticulum (ER) stress seems to be induced in KMS12PE and KMS11 cells treated with OSSL_325096