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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: J Cell Physiol. 2019 Jun 12;235(1):210–220. doi: 10.1002/jcp.28960

Figure 4.

Figure 4.

Calvarial osteoblast-enriched cells from Notch3tm1.1Ecan mutant (black bars) and wild type (white bars) littermate controls were isolated and cultured in the presence of anti-Notch3 NRR (N3NRR) or anti-ragweed control antibody (Ctrl) at 20 μg/ml for 1 week. Total RNA was extracted, and gene expression determined by qRT-PCR in the presence of specific primers. Data are expressed as Notch36691-TAATGA mutant, Hes1, Hey1, Hey2 and Tnfsf11 copy number corrected for Rpl38. Values are means ± SD; n = 4. *Significantly different between Notch3tm1.1Ecan mutant and wild type control cells, p < 0.05. #Significantly different between anti-Notch3 NRR and control antibody, p < 0.05.