Figure 3.

GWAS of RAD-seq trait values identifies a QTL on chromosome III. A) A Manhattan plot for GWAS using RAD-seq trait values (Table 1, Supplementary File 1) as input for 96 strains is presented. The horizontal red line indicates the Bonferroni-corrected threshold for statistical significance at a p-value of 0.05, and is 5.43 for this GWAS. A SNV at chromosome III: 292,584 is significantly associated with starvation survival. B) A genotype-by-phenotype plot for the marker SNV most significantly associated with variation in starvation resistance is presented. Each point represents a particular strain, with its genotype at the SNV and its RAD-seq trait value plotted. The most starvation-resistant strains, and a few others, have the alternative allele (C rather than T).