Skip to main content
. Author manuscript; available in PMC: 2019 Oct 7.
Published in final edited form as: Free Radic Biol Med. 2014 Apr 1;71:415–426. doi: 10.1016/j.freeradbiomed.2014.03.036

Fig. 7.

Fig. 7

Effects of H2O2 on the α1 Na/K-ATPase-associated Src. Purified Na/K-ATPase was first exposed to 10 mM H2O2 for 30 min, then to 1 U catalase to decompose the excess H2O2. Pretreated Na/K-ATPase was incubated with purified Src in the presence of 150 mM NaCl, 5 mM KCl, 100 μM vanadate, 10 μM ouabain, as indicated, and assayed for Src Tyr418 phosphorylation as described under Material and methods. Representative immunoblots and quantitative data from at least three independent experiments are shown. n=3–5. Values are expressed as the mean±SE. **p<0.01 compared with control.