Figure 2.
GABACR eIPSCs are more susceptible to Ca2+ buffering by EGTA-AM in early diabetes. Representative traces of GABACR eIPSCs from control (A) and diabetic (B) cells, before (black or gray traces) and after (blue trace) treatment with 50 μM EGTA-AM are shown. EGTA-AM significantly decreased the eIPSC D37 (C, P = 0.05 t-test) and charge transfer (D, P = 0.02 t-test) of diabetic cells (n = 8 cells from 5 mice) below the levels measured in control cells (n = 7 cells from 4 mice). All values are normalized to GABACR eIPSCs measured prior to applying EGTA-AM. Black bar: 1-ms stimulus. *P < 0.05.