Figure 3.

Electrically evoked GABA release onto rod bipolar cells GABA_CRs is more severely affected by Ca²⁺ buffering with EGTA-AM in early diabetes. The timecourse of GABA release onto rod bipolar cell GABA_CRs from control (A) and diabetic (B) cells was estimated by deconvolution analysis using the eIPSCs in Figure 2. Increased Ca²⁺-buffering with EGTA-AM reduced the timing (C, P = 0.03 t-test) and amount (D, P = 0.02 t-test) of vesicle release from diabetic cells (n = 8 cells from 5 mice) below the levels measured from control cells treated with EGTA-AM (n = 7 cells from 4 mice). All values are normalized to GABA release onto GABA_CRs measured prior to applying EGTA-AM. Black bar: 1-ms stimulus. *P < 0.05.