Figure 1.

GnRH stimulation increased oxidized PRDX isoforms in LβT2 cells. Cultured LβT2 or HeLa cells were incubated for 24 h in DMEM/10% FBS and then serum starved for 12 to 16 h. Cultures were subsequently treated with vehicle (Veh) or tonic 10 nM GnRH for 4 h or H₂O₂ for 30 min. Cells were lysed and subjected to SDS-PAGE and western blotted using antibodies against PRDX-SO_2/3 and β-actin. Band intensities for PRDX3-SO_2/3 and PRDX1/2-SO_2/3 were measured by quantitative chemiluminescence and normalized for β-actin. (a) A representative blot from three independent experiments. (b) Mean values ± SEM normalized to vehicles of four independent determinations. *Significant difference from the respective time-zero expression levels as determined by ANOVA and post hoc testing with Dunnett comparison with control test.