Fig. 2.

The 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities, and reducing power of rutin reacted in water or 80% ethanol with various concentration of HCl and reaction time. (A) 0.1 M HCl, (B) 0.5 M HCl, and (C) 1.0 M HCl in water, and (D) 0.1 M HCl, (E) 0.5 M HCl, and (F) 1.0 M HCl in 80% ethanol. The used concentration of hydrolyzed rutin is 30 μg/mL. The radical scavenging activities were expressed as Trolox equivalent antioxidant capacity (TEAC) and reducing power was expressed as absorbance at 700 nm. Each data represents the mean of triplicates. Error bars indicate the standard error (n=3). Mean values with different letters (a–f) are significantly different (P <0.05).