. 2019 Sep 30;24(3):327–337. doi: 10.3746/pnf.2019.24.3.327

Table 4.

AChE, BChE, and BACE1 inhibitory activities of four colored sweet peppers extracted using hexane, ethyl acetate, and 70% (v/v) aqueous ethanol

Sweet pepper extracts	AChE		BChE		% Inhibition of BACE1

	% Inhibition	IC₅₀ (g/L)	% Inhibition	IC₅₀ (g/L)
Hexane extracts
Green	43.55±3.62^b	52.90±5.07^c	10.22±1.22^a	ND	88.30±1.82^a
Red	25.38±0.15^c	106.40±5.09^b	6.35±0.66^b	ND	72.37±4.15^b
Orange	14.06±1.04^d	116.70±3.69^a	6.25±0.83^b	ND	56.33±4.42^c
Yellow	84.84±3.19^a	5.17±0.26^d	6.79±0.78^b	ND	66.99±2.81^b
Ethyl acetate extracts
Green	51.58±2.22^b	37.69±2.62^b	22.25±1.54^a	85.34±1.17	74.83±4.08^a
Red	24.75±1.04^c	77.63±0.99^c	10.91±0.80^b	ND	70.88±4.68^a
Orange	22.25±1.64^c	117.95±0.64^d	7.45±0.63^c	ND	60.18±6.61^b
Yellow	91.69±4.71^a	5.86±0.27^a	10.69±0.78^b	ND	69.58±3.84^a
70% (v/v) aqueous ethanol extracts
Green	39.00±3.75^b	59.35±2.62^b	32.25±2.55^a	62.64±2.13^c	58.73±3.72^a
Red	17.21±1.29^c	ND	29.79±2.53^ab	69.95±3.90^b	41.52±4.48^b
Orange	12.21±0.83^d	ND	24.50±1.99^c	81.53±3.20^a	39.18±0.79^b
Yellow	57.52±3.02^a	13.38±0.42^a	28.63±2.31^b	79.48±2.03^a	43.13±2.77^b

All data are expressed as mean±standard deviation.

The different letters (a–d) for each sweet pepper extracted using the same solvent and the same enzyme assay are significantly different at P<0.05 using one-way ANOVA followed by Tukey’s b post hoc test.

Final concentrations of all sweet pepper extracts (30.56 g dry weight/L) were used to determined IC50 values. AChE, acetylcholinesterase; BChE, butyrylcholinesterase; BACE1, β-secretase; IC50, the half maximal inhibitory concentration. ND, not detected.