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. Author manuscript; available in PMC: 2019 Nov 7.
Published in final edited form as: J Am Chem Soc. 2018 Oct 24;140(44):14567–14570. doi: 10.1021/jacs.8b08554

Figure 1.

Figure 1.

(A) Extracted ion chromatograms corresponding to masses of of 6-thio-2’-deoxyguanosine and 2-amino-6-(2,2,2-trifluoroethyl)-amino-2’-deoxyadenosine. (B) Restriction digest assay of a DNA duplex containing a single 6-thio-2’-deoxyguanosine, treated with 600 mM NH3 and 10 mM NaIO4 for 1 h at 45°C and subsequent digestion by SspI restriction enzyme (AATATT) and analysis by Native-PAGE. (C) Quantification of N to A transitions in ACTB mRNA sequencing reads. K562 cells were treated with 500 µM 6-TG for 1 h. Extracted RNA was treated with 600 mM TFEA and 10 mM NaIO4 for 1 h at 45°C.