KEY RESOURCE TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Experimental Models: Organisms/Strains
D. melanogaster: Oregon-R, wild-type laboratory stock		Flybase: FBst1000077
D. melanogaster: chromosomal deletion spanning hb w[1118]; Df(3R)BSC477/TM6C, Sb[1] cu[1]	Bloomington Drosophila Stock Center	Flybase: FBab0045343 BDSC: 24981
Oligonucleotides
smFISH probes for hb, see Table S1	This paper	N/A
smFISH probes for Kr, see Table S1	This paper	N/A
smFISH probes for kni, see Table S1	This paper	N/A
smFISH probes for gt, see Table S1	This paper	N/A
Software and Algorithms
FiSH Toolbox	Little et al. (2013)	N/A