FIGURE 5.

Immunohistochemical staining of paraffin embedded brain slices. Images obtained by fluorescence microscopy at the 48 h after injection time point from Vehicle (A) and Hyal (B) animals brain slices. The nuclear marker DAPI is shown in blue. Aggrecan, the component of perineuronal nets, is shown in green, neuronal marker NeuN is shuwn in red. The scale is 20 μm. We measured ECS area (C) and average neuronal body area (D) at the 2, 48, and 72 h after injection time points. Mann–Whitney test didn’t reveal any differences. The presence of aggrecan was measured by fluorescence intensity of green channel (E). There is significant decrease in aggrecan component at all the time points but the minimum was fixed 48 h after injections. At the time point of 72 h the level of aggrecan starts to restore. ^∗∗p < 0.005, ^****p < 0.0001.