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Flow cytometer’s setting
| Target molecules | Fluorochromes | Lasers and channels | Filters | |
|---|---|---|---|---|
| CD45 | BUV395 | Ultra Violet-B | 379/34 BP | - |
| CD90 | eFluor 450 | Violet-C | 450/50 BP | - |
| CD326 | BV510 | Violet-B | 525/50 BP | 505 LP |
| MHC-II I-A | FITC | Blue-B | 525/50 BP | - |
| Dead cells | 7-AAD | Blue-A | 710/50 BP | 695 LP |
| CD24 | PE | Yellow Green-E | 585/15 BP | - |
| T1a/Podoplanin (Pdpn) | Alexa Fluor 647 | Red-B | 660/20 BP | 750 LP |
| CD31 | APC-Fire 750 | Red-A | 780/60 BP | 735 LP |
BP: band pass, LP: long pass
