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. 2019 Oct 7;14(10):e0223572. doi: 10.1371/journal.pone.0223572

Fig 8. Percent damaged A549 cells after 1h lipid exchange at 26°C with or without a 2h recovery step at 37°C assayed with PI and Annexin V.

Fig 8

Exchange was carried out using a range of concentrations of: A) bSM; B) eSM; C) POPC; D) DOPC. Exchange was carried out in 6 cm diameter plates at 26°C with 1.5 ml of lipid plus 40mM MαCD. Recovery was carried out at 37°C in the same plates with 2 ml of complete growth medium. In untreated samples there was about 5% damaged cells. Mean and standard deviation from three experiments is shown.