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. 2019 Sep 25;15(9):e1007374. doi: 10.1371/journal.pcbi.1007374

Fig 2. New modelling approach developed in this study to explain vulnerable dynamics of Casp8 activation and fractional cell death upon DR stimulation.

Fig 2

(A) The DR clustering initiates the formation of a few RIPoptosomes in the closed proximity to each other where Casp8, in addition to cis-activation, can undergo trans-activation. (B) The initial stochastic Casp8 activation on the DISC/RIPoptosome platform is implemented by direct Gillespie stochastic simulation algorithm (SSA) which incorporates the molecular assembly of FADD, RIP1, RIP3, ProCasp8, cFLIPs/l proteins. (C) Complete deterministic system comprised of initiation ODE system (D) and feedback ODE system (E) used in the parameter scan and manual parameter adjustment. (D) Box represents the deterministic approximation of the population kinetics of Casp8 activation on the DISC/RIPoptosome platform. (E) Box represents the deterministic activation of the effector caspases, Casp3 and Casp6, which feedback to the rate of Casp8 activation before MOMP.