Skip to main content
. 2019 Sep 25;15(9):e1008380. doi: 10.1371/journal.pgen.1008380

Fig 9. Model.

Fig 9

The model depicts the relationship between the amount of CENP-A at centromeric chromatin at the onset of mitosis and the duration of mitosis. Cells that have a relative large pool of CENP-A at centromeres go through mitosis faster. The precise reason for this decreased mitotic timing is still unknown but we hypothesize that cells with sufficient CENP-A recruit SAC components more quickly or capture microtubules more efficiently to satisfy the SAC faster. This hypothesis is supported by our finding that the CENP-A loading factor CAL1 directly interact with the SAC component and RZZ subunit Zw10. More CAL1 may be able to not only recruit more CENP-A by priming centromeres and loading more efficiently CENP-A but also may recruit the RZZ complex and the SAC more efficiently. Vice versa, an active SAC may give the cell more time to recruit sufficient CENP-A via CAL1 to ensure that the cell does not progress further without having loaded sufficient CENP-A to the centromere.